
Ldl cholesterol esterase (Che) from Burkholderia stabilis (BsChe) is a homolog of well-characterized and industrially related bacterial triacylglycerol lipases (Lips). BsChe is a uncommon bacterial Lip enzyme that displays sensible Che exercise and is at the moment utilized in scientific purposes to find out complete serum levels of cholesterol. To analyze the sterol specificity of BsChe, we decided the X-ray construction of BsChe. We found a neighborhood structural change within the active-site cleft, which is perhaps associated to substrate binding and product launch.
We additionally carried out molecular docking research by utilizing the X-ray fashions of BsChe and ldl cholesterol linoleate (CLL), probably the most favorable substrate for BsChe. The outcomes confirmed that the sterol moieties of affordable CLL docking poses localized to a particular active-site cleft floor shaped by Leu266 and Ile287, that are unconserved amongst Burkholderia Lip homologs. Web site-directed mutagenesis recognized these residues as important for the Che exercise of BsChe, and Leu or Ile substitution conferred marked Che exercise to Burkholderia Lips.
Specifically, Burkholderia cepacia and Burkholderia ubonensis Lips with the V266L/L287I double mutation exhibited ~50-fold and 500-fold increased Che actions than these of the wild-type enzymes, respectively. These outcomes present new insights into the substrate-binding mechanisms and selectivities of bacterial Lips.
Pyronaridine: An replace of its pharmacological actions and mechanisms of motion

Bacterial triacylglycerol lipase is a potential cholesterol esterase: Identification of a key determinant for sterol-binding specificity
Built-in air cathode microbial gas cell-aerobic bioreactor set-up for enhanced bioelectrodegradation of azo dye Acid Blue 29
Jasmonic Acid for molecular biology, 95% |
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79238 | Sisco Laboratories | 25 Mg | EUR 120.9 |
Description: Part B |
L-Ascorbic Acid for molecular biology, 99.7% |
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14116 | Sisco Laboratories | 25 Gms | EUR 1.64 |
Description: Part A |
Acetic Acid Glacial for molecular biology, 99.9% |
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59788 | Sisco Laboratories | 100 ml | EUR 3.56 |
Description: Part A |
Trifluoroacetic Acid (TFA) for molecular biology, 99.9% |
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40801 | Sisco Laboratories | 100 ml | EUR 22.92 |
Description: Part A |
Casein, for Molecular Biology |
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MB279-100G | EWC Diagnostics | 1 unit | EUR 110.77 |
Description: Casein, for Molecular Biology |
Casein, for Molecular Biology |
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MB279-500G | EWC Diagnostics | 1 unit | EUR 387.65 |
Description: Casein, for Molecular Biology |
Sucrose for molecular biology |
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27580 | Sisco Laboratories | 500 Gms | EUR 4.11 |
Description: Part A |
5-Fluoroorotic Acid (5-FOA) for molecular biology, 98% |
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78510 | Sisco Laboratories | 250 Mg | EUR 29.78 |
Description: Part B |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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PD0252 | Bio Basic | 500g | EUR 192.59 |
Urea for molecular biology, 99.5% |
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21113 | Sisco Laboratories | 500 Gms | EUR 6.5 |
Description: Part A |
Xylene for molecular biology, 99.5% |
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45122 | Sisco Laboratories | 250 ml | EUR 2.81 |
Description: Part A |
Glycine for molecular biology, 99.5% |
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64072 | Sisco Laboratories | 100 Gms | EUR 1.64 |
Description: Part A |
Acetone for molecular biology, 99.8% |
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27498 | Sisco Laboratories | 500 ml | EUR 3.76 |
Description: Part A |
Methanol for molecular biology, 99.5% |
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96446 | Sisco Laboratories | 500 ml | EUR 6.23 |
Description: Part A |
Carbinol for molecular biology, 99.5% |
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34883 | Sisco Laboratories | 500 ml | EUR 6.23 |
Description: Part A |
Formamide for molecular biology, 99.5% |
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30349 | Sisco Laboratories | 500 ml | EUR 5.82 |
Description: Part A |
Imidazole for molecular biology, 99.5% |
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61510 | Sisco Laboratories | 25 Gms | EUR 5.47 |
Description: Part A |
Chloroform for molecular biology, 99.8% |
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96764 | Sisco Laboratories | 100 ml | EUR 3.08 |
Description: Part A |
Urea, suitable for molecular biology |
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GE1210 | Glentham Life Sciences | 500g | EUR 53.1 |
Urea, suitable for molecular biology |
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GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
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GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
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GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Triethylamine for molecular biology, 99.5% |
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55221 | Sisco Laboratories | 500 ml | EUR 4.11 |
Description: Part A |
Ficoll 400® for molecular biology |
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45460 | Sisco Laboratories | 5 Gms | EUR 15.6 |
Description: Part B |
Agarose UltraPhor for molecular biology |
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27813 | Sisco Laboratories | 10 Gms | EUR 46.5 |
Description: Part B |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
Triton X-100 for molecular biology |
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64518 | Sisco Laboratories | 100 ml | EUR 6.02 |
Description: Part A |
MOPS Buffer for molecular biology, 99% |
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66043 | Sisco Laboratories | 25 Gms | EUR 10.28 |
Description: Part B |
ACES Buffer for molecular biology, 99.5% |
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27907 | Sisco Laboratories | 5 Gms | EUR 5.4 |
Description: Part B |
Vitamin B12 for molecular biology, 97% |
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77472 | Sisco Laboratories | 250 Mg | EUR 5.78 |
Description: Part B |
Agarose Low EEO for molecular biology |
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36601 | Sisco Laboratories | 10 Gms | EUR 5.1 |
Description: Part B |
10X MOPS Buffer for molecular biology |
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68078 | Sisco Laboratories | 100 ml | EUR 4.5 |
Description: Part B |
CHAPS Buffer for molecular biology, 99.5% |
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21420 | Sisco Laboratories | 1 Gms | EUR 10.73 |
Description: Part B |
HEPES Buffer for molecular biology, 99.5% |
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16826 | Sisco Laboratories | 25 Gms | EUR 6.3 |
Description: Part B |
Sodium Azide for molecular biology, 99% |
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17782 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
PIPES Buffer for molecular biology, 99% |
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49159 | Sisco Laboratories | 100 Gms | EUR 26.03 |
Description: Part B |
Agarose High EEO for molecular biology |
|||
60645 | Sisco Laboratories | 25 Gms | EUR 13.8 |
Description: Part B |
Protamine Sulfate for molecular biology, 90-110% |
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78349 | Sisco Laboratories | 1 Gms | EUR 22.5 |
Description: Part B |
Oil Red O for molecular biology, 75% |
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23576 | Sisco Laboratories | 25 Gms | EUR 5.33 |
Description: Part B |
Sodium Chloride for molecular biology, 99.9% |
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33205 | Sisco Laboratories | 500 Gms | EUR 4.52 |
Description: Part A |
Agarose Medium EEO for molecular biology |
|||
10423 | Sisco Laboratories | 10 Gms | EUR 6.75 |
Description: Part B |
Isopropanol (IPA) for molecular biology, 99.8% |
|||
38445 | Sisco Laboratories | 100 ml | EUR 1.71 |
Description: Part A |
Acetonitrile (ACN) for molecular biology, 99.9% |
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62006 | Sisco Laboratories | 250 ml | EUR 4.93 |
Description: Part A |
Ethidium Bromide for molecular biology, 95% |
|||
93079 | Sisco Laboratories | 1 Gms | EUR 4.58 |
Description: Part A |
Ammonium Acetate for molecular biology, 98% |
|||
37829 | Sisco Laboratories | 100 Gms | EUR 2.12 |
Description: Part A |
Agarose Low Melting for molecular biology |
|||
32417 | Sisco Laboratories | 5 Gms | EUR 22.5 |
Description: Part B |
Potassium Acetate for molecular biology, 99.5% |
|||
96248 | Sisco Laboratories | 100 Gms | EUR 2.74 |
Description: Part A |
Ammonium Chloride for molecular biology, 99.5% |
|||
16992 | Sisco Laboratories | 500 Gms | EUR 4.52 |
Description: Part A |
Ammonium Sulphate for molecular biology, 99.5% |
|||
82126 | Sisco Laboratories | 250 Gms | EUR 4.93 |
Description: Part A |
5-Fluoroorotic Acid Hydrate (5-FOA Hydrate) for molecular biology, 98% |
|||
50958 | Sisco Laboratories | 250 Mg | EUR 31.5 |
Description: Part B |
Potassium Chloride for molecular biology, 99.5% |
|||
84984 | Sisco Laboratories | 500 Gms | EUR 6.16 |
Description: Part A |
Sodium Bicarbonate for molecular biology, 99.7% |
|||
36328 | Sisco Laboratories | 500 Gms | EUR 5.47 |
Description: Part A |
Glycerol (Glycerine) for molecular biology, 99.5% |
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62417 | Sisco Laboratories | 100 ml | EUR 2.05 |
Description: Part A |
Phenol Crystalline for molecular biology, 99.5% |
|||
17286 | Sisco Laboratories | 100 Gms | EUR 3.15 |
Description: Part A |
2-Mercaptoethanol for molecular biology, 99% |
|||
83759 | Sisco Laboratories | 100 ml | EUR 5.27 |
Description: Part A |
Acriflavine Neutral for molecular biology, 98% |
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42428 | Sisco Laboratories | 5 Gms | EUR 3.15 |
Description: Part B |
Dichloromethane (DCM) for molecular biology, 99.9% |
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41512 | Sisco Laboratories | 250 ml | EUR 3.22 |
Description: Part A |
Tetrahydrofuran (THF) for molecular biology, 99.8% |
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92562 | Sisco Laboratories | 250 ml | EUR 3.28 |
Description: Part A |
Dithioerythritol (DTE) for molecular biology, 99% |
|||
53384 | Sisco Laboratories | 1 Gms | EUR 9.53 |
Description: Part B |
Sodium chloride, suitable for molecular biology |
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GE0307 | Glentham Life Sciences | 1kg | EUR 41.33 |
Sodium chloride, suitable for molecular biology |
|||
GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
meta-Topolin (mT) for molecular biology, 98% |
|||
68913 | Sisco Laboratories | 25 Mg | EUR 17.48 |
Description: Part B |
HEPES Sodium Salt for molecular biology, 99% |
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32225 | Sisco Laboratories | 25 Gms | EUR 11.85 |
Description: Part B |
Sucrose, GlenBiol, suitable for molecular biology |
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GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Glycogen ex. Oyster for molecular biology, 85% |
|||
49740 | Sisco Laboratories | 1 Gms | EUR 7.5 |
Description: Part B |
D-Sorbitol Powder for molecular biology, 98% |
|||
99103 | Sisco Laboratories | 500 Gms | EUR 10.33 |
Description: Part A |
Acrylamide 3x cryst. for molecular biology, 99.9% |
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61346 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
Brilliant Blue G-250 for molecular biology |
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64222 | Sisco Laboratories | 5 Gms | EUR 4.65 |
Description: Part B |
Brilliant Blue R-250 for molecular biology |
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93473 | Sisco Laboratories | 5 Gms | EUR 4.35 |
Description: Part B |
Propidium Iodide (PI) for molecular biology, 95% |
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11195 | Sisco Laboratories | 10 Mg | EUR 17.63 |
Description: Part B |
Acriflavine Hydrochloride for molecular biology, 98.5%-105% |
|||
35188 | Sisco Laboratories | 5 Gms | EUR 4.05 |
Description: Part B |
Polysorbate 20 (Tween 20) for molecular biology |
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65296 | Sisco Laboratories | 100 Gms | EUR 10.81 |
Description: Part A |
Tris Buffer Superior for molecular biology, 99.9% |
|||
37969 | Sisco Laboratories | 100 Gms | EUR 7.59 |
Description: Part A |
Molecular Biology Grade Water for RT-PCR |
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ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
Description: Molecular Biology Grade Water for RT-PCR |
10X TE Buffer pH-8.0 for molecular biology |
|||
51782 | Sisco Laboratories | 100 ml | EUR 9.45 |
Description: Part B |
5-Fluoroorotic Acid Monohydrate (5-FOA Monohydrate) for molecular biology, 98% |
|||
11933 | Sisco Laboratories | 250 Mg | EUR 30.68 |
Description: Part B |
10X TBE Buffer pH-8.3 for molecular biology |
|||
83170 | Sisco Laboratories | 200 ml | EUR 4.95 |
Description: Part B |
Tris Buffer AR, ACS for molecular biology, 99.9% |
|||
71033 | Sisco Laboratories | 100 Gms | EUR 6.02 |
Description: Part A |
10X TAE Buffer pH-8.3 for molecular biology |
|||
71989 | Sisco Laboratories | 100 ml | EUR 4.43 |
Description: Part B |
Acrylamide 40% aq. solution for molecular biology |
|||
29787 | Sisco Laboratories | 250 ml | EUR 20.53 |
Description: Part A |
MES Monohydrate Buffer for molecular biology, 99% |
|||
26854 | Sisco Laboratories | 25 Gms | EUR 11.03 |
Description: Part B |
Glutathione Reduced (GSH) for molecular biology, 99.5% |
|||
11514 | Sisco Laboratories | 1 Gms | EUR 5.55 |
Description: Part B |
DL-Dithiothreitol (DTT) for molecular biology, 98% |
|||
17315 | Sisco Laboratories | 1 Gms | EUR 5.18 |
Description: Part B |
Ammonium Persulphate (APS) for molecular biology, 99% |
|||
65553 | Sisco Laboratories | 25 Gms | EUR 1.64 |
Description: Part A |
Sodium Acetate Anhydrous for molecular biology, 99% |
|||
22342 | Sisco Laboratories | 250 Gms | EUR 3.56 |
Description: Part A |
Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8% |
|||
24075 | Sisco Laboratories | 100 ml | EUR 6.02 |
Description: Part A |
Sodium Acetate Trihydrate for molecular biology, 99.5% |
|||
88035 | Sisco Laboratories | 250 Gms | EUR 2.33 |
Description: Part A |
Cesium Sulphate extrapure for molecular biology, 99.9% |
|||
57455 | Sisco Laboratories | 10 Gms | EUR 7.5 |
Description: Part B |
Glutathione Oxidized (GSSG) for molecular biology, 99.5% |
|||
22151 | Sisco Laboratories | 250 Mg | EUR 22.5 |
Description: Part B |
Guanidine Thiocyanate (GTC) for molecular biology, 99% |
|||
80272 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
Cesium Chloride ultrapure for molecular biology, 99.9% |
|||
32355 | Sisco Laboratories | 25 Gms | EUR 18 |
Description: Part B |
Sucrose, GlenBiol™, suitable for molecular biology |
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GC3201 | Glentham Life Sciences | 1kg | EUR 41.25 |
Sucrose, GlenBiol™, suitable for molecular biology |
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GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780 | Glentham Life Sciences | 2500ml | EUR 239.63 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Calcium Chloride Dihydrate for molecular biology, 99.5% |
|||
97080 | Sisco Laboratories | 100 Gms | EUR 2.12 |
Description: Part A |
Diethyl Pyrocarbonate (DEPC) for molecular biology, 99% |
|||
46791 | Sisco Laboratories | 5 ml | EUR 9 |
Description: Part B |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659 | Glentham Life Sciences | 500ml | EUR 230.38 |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
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GS9663 | Glentham Life Sciences | 100ml | EUR 46.83 |
Formamide, GlenBiol™, suitable for molecular biology |
|||
GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
20X SSC Buffer pH 6.9-7.1 for molecular biology |
|||
12590 | Sisco Laboratories | 200 ml | EUR 2.48 |
Description: Part B |
Bromocresol Purple Sodium Salt for molecular biology |
|||
24836 | Sisco Laboratories | 5 Gms | EUR 2.05 |
Description: Part A |
Guanidine Hydrochloride (GHC) for molecular biology, 99.5% |
|||
45539 | Sisco Laboratories | 100 Gms | EUR 4.79 |
Description: Part A |
Polyethylene Glycol 8000 (PEG 8000) for molecular biology |
|||
54866 | Sisco Laboratories | 500 Gms | EUR 11.97 |
Description: Part A |
10X Tris Buffered Saline (TBS) for molecular biology |
|||
83471 | Sisco Laboratories | 500 ml | EUR 14.7 |
Description: Part B |
Magnesium Acetate Tetrahydrate for molecular biology, 99% |
|||
50488 | Sisco Laboratories | 100 Gms | EUR 2.74 |
Description: Part A |
Magnesium Chloride Hexahydrate for molecular biology, 99.5% |
|||
91417 | Sisco Laboratories | 250 Gms | EUR 4.45 |
Description: Part A |
Magnesium Sulphate Heptahydrate for molecular biology, 99.5% |
|||
74440 | Sisco Laboratories | 500 Gms | EUR 6.16 |
Description: Part A |
Tris Hydrochloride (Tris HCl) for molecular biology, 99% |
|||
89781 | Sisco Laboratories | 100 Gms | EUR 9.37 |
Description: Part A |
L-Lysine Monohydrate (base) for molecular biology, 99% |
|||
45976 | Sisco Laboratories | 25 Gms | EUR 37.63 |
Description: Part A |
Agarose Low EEO Superior Grade for molecular biology |
|||
23287 | Sisco Laboratories | 10 Gms | EUR 6 |
Description: Part B |
OORA00229-1L - Molecular Biology Grade UltraPure Water |
|||
OORA00229-1L | Aviva Systems Biology | 1L | EUR 149 |
OORA00230-1L - Molecular Biology Grade UltraPure Water |
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OORA00230-1L | Aviva Systems Biology | 1L | EUR 279 |
DTT (Molecular Biology Grade) |
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CE131 | GeneOn | 5 g | EUR 44 |
DTT (Molecular Biology Grade) |
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CE132 | GeneOn | 10 g | EUR 88 |
DTT (Molecular Biology Grade) |
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CE133 | GeneOn | 25 g | EUR 170 |
NAD (Molecular Biology Grade) |
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CE196 | GeneOn | 1 g | EUR 34 |
NAD (Molecular Biology Grade) |
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CE197 | GeneOn | 5 g | EUR 137 |
NBT (Molecular Biology Grade) |
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CE209 | GeneOn | 1 g | EUR 96 |
NBT (Molecular Biology Grade) |
|||
CE210 | GeneOn | 5 g | EUR 345 |
10X Tris-Glycine-SDS Buffer for molecular biology |
|||
57806 | Sisco Laboratories | 200 ml | EUR 3.6 |
Description: Part B |
N,N-Dimethylformamide (DMF) for molecular biology, 99.9% |
|||
24017 | Sisco Laboratories | 100 ml | EUR 3.08 |
Description: Part A |
Cesium Chloride extrapure AR for molecular biology, 99.9% |
|||
22966 | Sisco Laboratories | 25 Gms | EUR 14.63 |
Description: Part B |
10X Tris-Tricine-SDS Buffer for molecular biology |
|||
37852 | Sisco Laboratories | 500 ml | EUR 9.08 |
Description: Part B |
EDTA Disodium Salt Dihydrate for molecular biology, 99.5% |
|||
43272 | Sisco Laboratories | 100 Gms | EUR 2.26 |
Description: Part A |
DMSO, Molecular Biology Grade |
|||
40470006-1 | Bio-WORLD | 100 mL | EUR 108.14 |
DMSO, Molecular Biology Grade |
|||
40470006-2 | Bio-WORLD | 250 mL | EUR 182.67 |
DMSO, Molecular Biology Grade |
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40470006-3 | Bio-WORLD | 500 mL | EUR 342.49 |
EGTA, Molecular Biology Grade |
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40500028-2 | Bio-WORLD | 50 g | EUR 130.53 |
EGTA, Molecular Biology Grade |
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40500028-3 | Bio-WORLD | 100 g | EUR 217.78 |
EGTA, Molecular Biology Grade |
|||
40500028-4 | Bio-WORLD | 500 g | EUR 734.55 |
EGTA, Molecular Biology Grade |
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40500028-5 | Bio-WORLD | 1 kg | EUR 1119.67 |