
The place classical epidemiology has confirmed to be insufficient for surveillance and management of foodborne pathogens, molecular epidemiology, utilizing genomic typing strategies, can add worth. Nevertheless, the evaluation of entire genome sequencing (WGS) information varies extensively and isn’t but absolutely harmonised. We used genomic information on 494 Listeria monocytogenes isolates from ready-to-eat meals merchandise and meals processing environments deposited within the pressure assortment of the German Nationwide Reference Laboratory to check varied procedures for WGS information evaluation and to judge compatibility of outcomes.
Two totally different core genome multilocus sequence typing (cgMLST) schemes, totally different reference genomes in single nucleotide polymorphism (SNP) evaluation and business in addition to open-source software program have been in contrast. Correlation of allele distances from the totally different cgMLST approaches was excessive, starting from 0.97 to 1, and unified thresholds yielded greater clustering concordance than scheme-specific thresholds. The variety of detected SNP variations might be elevated as much as an element of three.9 utilizing a selected reference genome in contrast with a normal one. Moreover, particular reference genomes improved comparability of SNP evaluation outcomes obtained utilizing totally different software program instruments. The usage of a closed or a draft particular reference genome didn’t make a distinction.
The harmonisation of WGS information evaluation will lastly assure seamless information alternate, however, within the meantime, data on threshold values that result in comparable clustering of isolates by totally different strategies might enhance communication between laboratories. We subsequently established a translation code between generally utilized cgMLST and SNP strategies primarily based on optimised clustering concordances. This code can work as a primary filter to establish WGS-based typing matches ensuing from totally different strategies, which opens up a brand new perspective for information alternate and thereby accelerates time-critical analyses, equivalent to in outbreak investigations.
Antibacterial and Antifungal Properties of Composite Polyethylene Supplies Bolstered with Neem and Turmeric
An Autism-Related Mutation Impairs Neuroligin-Four Glycosylation and Enhances Excitatory Synaptic Transmission in Human Neurons
Urea, suitable for molecular biology |
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GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Urea, suitable for molecular biology |
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GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
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GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Sodium chloride, suitable for molecular biology |
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GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
Tris (Molecular Biology Grade) |
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CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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CE239 | GeneOn | 5 kg | EUR 535.2 |
Sucrose, GlenBiol, suitable for molecular biology |
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GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Sucrose, GlenBiol™, suitable for molecular biology |
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GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
Formamide, GlenBiol™, suitable for molecular biology |
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GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
NAD (Molecular Biology Grade) |
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CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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CE210 | GeneOn | 5 g | EUR 360 |
DTT (Molecular Biology Grade) |
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CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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CE133 | GeneOn | 25 g | EUR 243.6 |
BCIP (Molecular Biology Grade) |
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CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
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CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
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CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
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CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
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CE119 | GeneOn | 100 mg | EUR 382.8 |
100mL Molecular Biology Grade |
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46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 74.4 |
500mL Molecular Biology Grade |
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46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 138 |
HEPES (Molecular Biology Grade) |
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CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
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CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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CE244 | GeneOn | 1 l | EUR 67.2 |
CHAPS (Molecular Biology Grade) |
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CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
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CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
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CE116 | GeneOn | 25 g | EUR 492 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
Tween20 (Molecular Biology Grade) |
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CE242 | GeneOn | 1 l | EUR 106.8 |
Glycine (Molecular Biology Grade) |
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CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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CE159 | GeneOn | 5 kg | EUR 228 |
Lysozyme (Molecular Biology Grade) |
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CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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CE189 | GeneOn | 10 g | EUR 247.2 |
Formamide deionized (Molecular Biology Grade) |
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CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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CE146 | GeneOn | 1 l | EUR 120 |
Dimethylformamide, GlenBiol™, suitable for molecular biology |
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GS6580-2500 | Glentham Life Sciences | 2500 | EUR 107.3 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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GK8512-1L | Glentham Life Sciences | 1 l | EUR 92.4 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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GS0247-1 | Glentham Life Sciences | 1 | EUR 40.7 |
Acetonitrile 50, GlenBiol™, suitable for molecular biology |
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GS0247-2500 | Glentham Life Sciences | 2500 | EUR 61.3 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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GS0969-1 | Glentham Life Sciences | 1 | EUR 47 |
Acetonitrile 10, GlenBiol™, suitable for molecular biology |
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GS0969-2500 | Glentham Life Sciences | 2500 | EUR 73.9 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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GS8649-1 | Glentham Life Sciences | 1 | EUR 43.1 |
Acetonitrile 30, GlenBiol™, suitable for molecular biology |
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GS8649-2500 | Glentham Life Sciences | 2500 | EUR 66.5 |
Water, distilled, GlenBiol™, suitable for molecular biology |
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GK8512-1 | Glentham Life Sciences | 1 | EUR 60.1 |
Phenol, (Carbolic acid) Double distilled for Molecular Biology |
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PD0252 | Bio Basic | 500g | EUR 192.59 |
Ammonia solution, GlenBiol™, suitable for molecular biology |
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GS8853-100 | Glentham Life Sciences | 100 | EUR 91.2 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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GE6258-100 | Glentham Life Sciences | 100 | EUR 150.4 |
Agarose, low EEO, GlenBiol™, suitable for molecular biology |
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GE6258-25 | Glentham Life Sciences | 25 | EUR 60.1 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE234 | GeneOn | 250 g | EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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CE236 | GeneOn | 1 kg | EUR 223.2 |
1L Molecular Biology Grade Water |
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46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 196.8 |
Tween 20, Molecular Biology Grade |
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T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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T9100-100 | GenDepot | 1L | EUR 160.8 |
TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology |
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GE6710-1 | Glentham Life Sciences | 1 | EUR 67.9 |
TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology |
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GE6710-250 | Glentham Life Sciences | 250 | EUR 22.7 |
Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g |
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41028-100G | Biotium | 100G | EUR 266.4 |
Description: Minimum order quantity: 1 unit of 100G |
D(+)-Sucrose (Molecular Biology Grade) |
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CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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CE226 | GeneOn | 5 kg | EUR 207.6 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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GX7489-1 | Glentham Life Sciences | 1 | EUR 22.7 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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GX7489-2500 | Glentham Life Sciences | 2500 | EUR 45.2 |
Tris-EDTA buffer solution (10X, suitable for molecular biology, pH 8.0) |
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GX7489-500 | Glentham Life Sciences | 500 | EUR 15.9 |
MOPS buffer (Molecular Biology Grade) |
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CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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CE195 | GeneOn | 250 g | EUR 169.2 |
Glycerol 87 % (Molecular Biology Grade) |
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CE154 | GeneOn | 1 l | EUR 93.6 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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CE121 | GeneOn | 500 ml | EUR 110.4 |
Water, Ultrapure Molecular Biology Grade |
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41024-4L | Biotium | 4L | EUR 145.2 |
Description: Minimum order quantity: 1 unit of 4L |
Sodium chloride (Molecular Biology Grade) |
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CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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CE207 | GeneOn | 5 kg | EUR 123.6 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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CE111 | GeneOn | 250 g | EUR 259.2 |
Urea Crystalline (Molecular Biology Grade) |
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CE167 | GeneOn | 1 kg | EUR 72 |
Urea Crystalline (Molecular Biology Grade) |
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CE168 | GeneOn | 5 kg | EUR 181.2 |
Ammonium sulfate (Molecular Biology Grade) |
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CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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CE107 | GeneOn | 5 kg | EUR 153.6 |
TRIS-Glycine-SDS Buffer 10X, GlenBiol™, suitable for molecular biology |
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GE9118-1 | Glentham Life Sciences | 1 | EUR 75.3 |
TRIS-Glycine-SDS Buffer 10X, GlenBiol™, suitable for molecular biology |
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GE9118-250 | Glentham Life Sciences | 250 | EUR 30.1 |
SSC Buffer (20X) (Molecular Biology Grade) |
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CE229 | GeneOn | 1 l | EUR 86.4 |
3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology |
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GS6015-100 | Glentham Life Sciences | 100 | EUR 74.5 |
3-Methylbutan-1-ol, GlenBiol™, suitable for molecular biology |
|||
GS6015-500 | Glentham Life Sciences | 500 | EUR 301.4 |
XTT sodium salt (Molecular Biology Grade) |
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CE250 | GeneOn | 100 mg | EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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CE251 | GeneOn | 500 mg | EUR 612 |
Glycerol waterfree (Molecular Biology Grade) |
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CE155 | GeneOn | 500 ml | EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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CE156 | GeneOn | 1 l | EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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CE157 | GeneOn | 2.5 l | EUR 170.4 |
NADP - sodium salt (Molecular Biology Grade) |
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CE200 | GeneOn | 250 mg | EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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CE201 | GeneOn | 1 g | EUR 190.8 |
Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology |
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GS7181-100 | Glentham Life Sciences | 100 | EUR 143.3 |
Di-iso-propylethylamine, GlenBiol™, suitable for molecular biology |
|||
GS7181-500 | Glentham Life Sciences | 500 | EUR 474.2 |
Guanidine Thiocyanate (Molecular Biology Grade) |
|||
CE164 | GeneOn | 100 g | EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
|||
CE165 | GeneOn | 500 g | EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
|||
CE166 | GeneOn | 1 kg | EUR 307.2 |
NADH - Disodium salt (Molecular Biology Grade) |
|||
CE198 | GeneOn | 1 g | EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
|||
CE199 | GeneOn | 5 g | EUR 244.8 |
Ammonium acetate 7.5M solution, GlenBiol™, suitable for molecular biology |
|||
GX5459-1 | Glentham Life Sciences | 1 | EUR 271.2 |
Ammonium acetate 7.5M solution, GlenBiol™, suitable for molecular biology |
|||
GX5459-250 | Glentham Life Sciences | 250 | EUR 90.4 |
N-Methyl-2-pyrrolidone, GlenBiol™, suitable for molecular biology |
|||
GS5724-2500 | Glentham Life Sciences | 2500 | EUR 126.3 |
Ammonium acetate 5M solution, GlenBiol™, suitable for molecular biology |
|||
GX2678-1 | Glentham Life Sciences | 1 | EUR 271.2 |
Ammonium acetate 5M solution, GlenBiol™, suitable for molecular biology |
|||
GX2678-250 | Glentham Life Sciences | 250 | EUR 90.4 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
|||
CE163 | GeneOn | 1 kg | EUR 352.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
|||
CE160 | GeneOn | 100 g | EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
|||
CE161 | GeneOn | 250 g | EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
|||
CE162 | GeneOn | 500 g | EUR 232.8 |
Yeast extract powder (Molecular Biology Grade) |
|||
CE169 | GeneOn | 500 g | EUR 133.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
|||
CE148 | GeneOn | 500 g | EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
|||
CE149 | GeneOn | 1 kg | EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
|||
CE150 | GeneOn | 5 kg | EUR 180 |
Hyaluronidase Grade I (Molecular Biology Grade) |
|||
CE174 | GeneOn | 1 g | EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
|||
CE175 | GeneOn | 5 g | EUR 920.4 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE204 | GeneOn | 500 mg | EUR 374.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
|||
CE100 | GeneOn | 50 g | EUR 128.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
|||
CE101 | GeneOn | 100 g | EUR 193.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
|||
CE102 | GeneOn | 250 g | EUR 387.6 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
|||
CE103 | GeneOn | 500 g | EUR 656.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
|||
CE104 | GeneOn | 1 kg | EUR 1162.8 |