The place classical epidemiology has confirmed to be insufficient for surveillance and management of foodborne pathogens, molecular epidemiology, utilizing genomic typing strategies, can add worth. Nevertheless, the evaluation of entire genome sequencing (WGS) information varies extensively and isn’t but absolutely harmonised. We used genomic information on 494 Listeria monocytogenes isolates from ready-to-eat meals merchandise and meals processing environments deposited within the pressure assortment of the German Nationwide Reference Laboratory to check varied procedures for WGS information evaluation and to judge compatibility of outcomes.
Two totally different core genome multilocus sequence typing (cgMLST) schemes, totally different reference genomes in single nucleotide polymorphism (SNP) evaluation and business in addition to open-source software program have been in contrast. Correlation of allele distances from the totally different cgMLST approaches was excessive, starting from 0.97 to 1, and unified thresholds yielded greater clustering concordance than scheme-specific thresholds. The variety of detected SNP variations might be elevated as much as an element of three.9 utilizing a selected reference genome in contrast with a normal one. Moreover, particular reference genomes improved comparability of SNP evaluation outcomes obtained utilizing totally different software program instruments. The usage of a closed or a draft particular reference genome didn’t make a distinction.
The harmonisation of WGS information evaluation will lastly assure seamless information alternate, however, within the meantime, data on threshold values that result in comparable clustering of isolates by totally different strategies might enhance communication between laboratories. We subsequently established a translation code between generally utilized cgMLST and SNP strategies primarily based on optimised clustering concordances. This code can work as a primary filter to establish WGS-based typing matches ensuing from totally different strategies, which opens up a brand new perspective for information alternate and thereby accelerates time-critical analyses, equivalent to in outbreak investigations.
Antibacterial and Antifungal Properties of Composite Polyethylene Supplies Bolstered with Neem and Turmeric
An Autism-Related Mutation Impairs Neuroligin-Four Glycosylation and Enhances Excitatory Synaptic Transmission in Human Neurons
TritonX-100 (Molecular Biology Grade) |
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CE241 | GeneOn | 1 l | EUR 79.2 |
Casein, for Molecular Biology |
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MB279-100G | EWC Diagnostics | 1 unit | EUR 110.77 |
Description: Casein, for Molecular Biology |
Casein, for Molecular Biology |
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MB279-500G | EWC Diagnostics | 1 unit | EUR 387.65 |
Description: Casein, for Molecular Biology |
Sucrose for molecular biology |
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27580 | Sisco Laboratories | 500 Gms | EUR 4.11 |
Description: Part A |
Triethylamine for molecular biology, 99.5% |
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55221 | Sisco Laboratories | 500 ml | EUR 4.11 |
Description: Part A |
Urea for molecular biology, 99.5% |
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21113 | Sisco Laboratories | 500 Gms | EUR 6.5 |
Description: Part A |
Xylene for molecular biology, 99.5% |
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45122 | Sisco Laboratories | 250 ml | EUR 2.81 |
Description: Part A |
Acetone for molecular biology, 99.8% |
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27498 | Sisco Laboratories | 500 ml | EUR 3.76 |
Description: Part A |
Glycine for molecular biology, 99.5% |
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64072 | Sisco Laboratories | 100 Gms | EUR 1.64 |
Description: Part A |
Formamide for molecular biology, 99.5% |
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30349 | Sisco Laboratories | 500 ml | EUR 5.82 |
Description: Part A |
Carbinol for molecular biology, 99.5% |
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34883 | Sisco Laboratories | 500 ml | EUR 6.23 |
Description: Part A |
Methanol for molecular biology, 99.5% |
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96446 | Sisco Laboratories | 500 ml | EUR 6.23 |
Description: Part A |
Imidazole for molecular biology, 99.5% |
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61510 | Sisco Laboratories | 25 Gms | EUR 5.47 |
Description: Part A |
Chloroform for molecular biology, 99.8% |
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96764 | Sisco Laboratories | 100 ml | EUR 3.08 |
Description: Part A |
Urea, suitable for molecular biology |
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GE1210 | Glentham Life Sciences | 500g | EUR 53.1 |
Urea, suitable for molecular biology |
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GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
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GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
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GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Ficoll 400® for molecular biology |
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45460 | Sisco Laboratories | 5 Gms | EUR 15.6 |
Description: Part B |
Agarose UltraPhor for molecular biology |
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27813 | Sisco Laboratories | 10 Gms | EUR 46.5 |
Description: Part B |
Boric Acid for molecular biology, 99.5% |
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22311 | Sisco Laboratories | 250 Gms | EUR 4.04 |
Description: Part A |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
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MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
ACES Buffer for molecular biology, 99.5% |
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27907 | Sisco Laboratories | 5 Gms | EUR 5.4 |
Description: Part B |
MOPS Buffer for molecular biology, 99% |
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66043 | Sisco Laboratories | 25 Gms | EUR 10.28 |
Description: Part B |
Vitamin B12 for molecular biology, 97% |
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77472 | Sisco Laboratories | 250 Mg | EUR 5.78 |
Description: Part B |
HEPES Buffer for molecular biology, 99.5% |
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16826 | Sisco Laboratories | 25 Gms | EUR 6.3 |
Description: Part B |
Sodium Azide for molecular biology, 99% |
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17782 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
CHAPS Buffer for molecular biology, 99.5% |
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21420 | Sisco Laboratories | 1 Gms | EUR 10.73 |
Description: Part B |
Agarose Low EEO for molecular biology |
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36601 | Sisco Laboratories | 10 Gms | EUR 5.1 |
Description: Part B |
PIPES Buffer for molecular biology, 99% |
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49159 | Sisco Laboratories | 100 Gms | EUR 26.03 |
Description: Part B |
10X MOPS Buffer for molecular biology |
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68078 | Sisco Laboratories | 100 ml | EUR 4.5 |
Description: Part B |
Tris Buffer Superior for molecular biology, 99.9% |
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37969 | Sisco Laboratories | 100 Gms | EUR 7.59 |
Description: Part A |
Agarose High EEO for molecular biology |
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60645 | Sisco Laboratories | 25 Gms | EUR 13.8 |
Description: Part B |
Jasmonic Acid for molecular biology, 95% |
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79238 | Sisco Laboratories | 25 Mg | EUR 120.9 |
Description: Part B |
Tris Buffer AR, ACS for molecular biology, 99.9% |
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71033 | Sisco Laboratories | 100 Gms | EUR 6.02 |
Description: Part A |
Oil Red O for molecular biology, 75% |
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23576 | Sisco Laboratories | 25 Gms | EUR 5.33 |
Description: Part B |
Protamine Sulfate for molecular biology, 90-110% |
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78349 | Sisco Laboratories | 1 Gms | EUR 22.5 |
Description: Part B |
Agarose Medium EEO for molecular biology |
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10423 | Sisco Laboratories | 10 Gms | EUR 6.75 |
Description: Part B |
Sodium Chloride for molecular biology, 99.9% |
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33205 | Sisco Laboratories | 500 Gms | EUR 4.52 |
Description: Part A |
Isopropanol (IPA) for molecular biology, 99.8% |
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38445 | Sisco Laboratories | 100 ml | EUR 1.71 |
Description: Part A |
Agarose Low Melting for molecular biology |
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32417 | Sisco Laboratories | 5 Gms | EUR 22.5 |
Description: Part B |
Ammonium Acetate for molecular biology, 98% |
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37829 | Sisco Laboratories | 100 Gms | EUR 2.12 |
Description: Part A |
Acetonitrile (ACN) for molecular biology, 99.9% |
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62006 | Sisco Laboratories | 250 ml | EUR 4.93 |
Description: Part A |
Ethidium Bromide for molecular biology, 95% |
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93079 | Sisco Laboratories | 1 Gms | EUR 4.58 |
Description: Part A |
RNase/DNase free water, for molecular biology (10 x 1.5ml) |
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Z-RNase/DNase-free-water | Novacyt Group | n/a | EUR 62 |
Trifluoroacetic Acid (TFA) for molecular biology, 99.9% |
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40801 | Sisco Laboratories | 100 ml | EUR 22.92 |
Description: Part A |
Ammonium Chloride for molecular biology, 99.5% |
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16992 | Sisco Laboratories | 500 Gms | EUR 4.52 |
Description: Part A |
Ammonium Sulphate for molecular biology, 99.5% |
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82126 | Sisco Laboratories | 250 Gms | EUR 4.93 |
Description: Part A |
Potassium Acetate for molecular biology, 99.5% |
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96248 | Sisco Laboratories | 100 Gms | EUR 2.74 |
Description: Part A |
Sodium Acetate Trihydrate for molecular biology, 99.5% |
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88035 | Sisco Laboratories | 250 Gms | EUR 2.33 |
Description: Part A |
Phenol Crystalline for molecular biology, 99.5% |
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17286 | Sisco Laboratories | 100 Gms | EUR 3.15 |
Description: Part A |
Sodium Bicarbonate for molecular biology, 99.7% |
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36328 | Sisco Laboratories | 500 Gms | EUR 5.47 |
Description: Part A |
Glycerol (Glycerine) for molecular biology, 99.5% |
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62417 | Sisco Laboratories | 100 ml | EUR 2.05 |
Description: Part A |
Potassium Chloride for molecular biology, 99.5% |
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84984 | Sisco Laboratories | 500 Gms | EUR 6.16 |
Description: Part A |
Dichloromethane (DCM) for molecular biology, 99.9% |
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41512 | Sisco Laboratories | 250 ml | EUR 3.22 |
Description: Part A |
Acriflavine Neutral for molecular biology, 98% |
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42428 | Sisco Laboratories | 5 Gms | EUR 3.15 |
Description: Part B |
2-Mercaptoethanol for molecular biology, 99% |
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83759 | Sisco Laboratories | 100 ml | EUR 5.27 |
Description: Part A |
Tetrahydrofuran (THF) for molecular biology, 99.8% |
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92562 | Sisco Laboratories | 250 ml | EUR 3.28 |
Description: Part A |
Dithioerythritol (DTE) for molecular biology, 99% |
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53384 | Sisco Laboratories | 1 Gms | EUR 9.53 |
Description: Part B |
Tris Hydrochloride (Tris HCl) for molecular biology, 99% |
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89781 | Sisco Laboratories | 100 Gms | EUR 9.37 |
Description: Part A |
Sodium chloride, suitable for molecular biology |
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GE0307 | Glentham Life Sciences | 1kg | EUR 41.33 |
Sodium chloride, suitable for molecular biology |
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GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
L-Ascorbic Acid for molecular biology, 99.7% |
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14116 | Sisco Laboratories | 25 Gms | EUR 1.64 |
Description: Part A |
HEPES Sodium Salt for molecular biology, 99% |
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32225 | Sisco Laboratories | 25 Gms | EUR 11.85 |
Description: Part B |
meta-Topolin (mT) for molecular biology, 98% |
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68913 | Sisco Laboratories | 25 Mg | EUR 17.48 |
Description: Part B |
10X Tris Buffered Saline (TBS) for molecular biology |
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83471 | Sisco Laboratories | 500 ml | EUR 14.7 |
Description: Part B |
Sucrose, GlenBiol, suitable for molecular biology |
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GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Glycogen ex. Oyster for molecular biology, 85% |
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49740 | Sisco Laboratories | 1 Gms | EUR 7.5 |
Description: Part B |
Propidium Iodide (PI) for molecular biology, 95% |
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11195 | Sisco Laboratories | 10 Mg | EUR 17.63 |
Description: Part B |
Acetic Acid Glacial for molecular biology, 99.9% |
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59788 | Sisco Laboratories | 100 ml | EUR 3.56 |
Description: Part A |
Acrylamide 3x cryst. for molecular biology, 99.9% |
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61346 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
Brilliant Blue G-250 for molecular biology |
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64222 | Sisco Laboratories | 5 Gms | EUR 4.65 |
Description: Part B |
Brilliant Blue R-250 for molecular biology |
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93473 | Sisco Laboratories | 5 Gms | EUR 4.35 |
Description: Part B |
D-Sorbitol Powder for molecular biology, 98% |
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99103 | Sisco Laboratories | 500 Gms | EUR 10.33 |
Description: Part A |
Acriflavine Hydrochloride for molecular biology, 98.5%-105% |
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35188 | Sisco Laboratories | 5 Gms | EUR 4.05 |
Description: Part B |
Polysorbate 20 (Tween 20) for molecular biology |
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65296 | Sisco Laboratories | 100 Gms | EUR 10.81 |
Description: Part A |
10X Tris-Tricine-SDS Buffer for molecular biology |
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37852 | Sisco Laboratories | 500 ml | EUR 9.08 |
Description: Part B |
10X Tris-Glycine-SDS Buffer for molecular biology |
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57806 | Sisco Laboratories | 200 ml | EUR 3.6 |
Description: Part B |
10X TE Buffer pH-8.0 for molecular biology |
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51782 | Sisco Laboratories | 100 ml | EUR 9.45 |
Description: Part B |
10X TAE Buffer pH-8.3 for molecular biology |
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71989 | Sisco Laboratories | 100 ml | EUR 4.43 |
Description: Part B |
10X TBE Buffer pH-8.3 for molecular biology |
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83170 | Sisco Laboratories | 200 ml | EUR 4.95 |
Description: Part B |
MES Monohydrate Buffer for molecular biology, 99% |
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26854 | Sisco Laboratories | 25 Gms | EUR 11.03 |
Description: Part B |
Acrylamide 40% aq. solution for molecular biology |
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29787 | Sisco Laboratories | 250 ml | EUR 20.53 |
Description: Part A |
Molecular Biology Grade Water for RT-PCR |
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ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
Description: Molecular Biology Grade Water for RT-PCR |
Glutathione Reduced (GSH) for molecular biology, 99.5% |
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11514 | Sisco Laboratories | 1 Gms | EUR 5.55 |
Description: Part B |
DL-Dithiothreitol (DTT) for molecular biology, 98% |
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17315 | Sisco Laboratories | 1 Gms | EUR 5.18 |
Description: Part B |
Sodium Acetate Anhydrous for molecular biology, 99% |
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22342 | Sisco Laboratories | 250 Gms | EUR 3.56 |
Description: Part A |
Dimethyl Sulphoxide (DMSO) for molecular biology, 99.8% |
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24075 | Sisco Laboratories | 100 ml | EUR 6.02 |
Description: Part A |
Ammonium Persulphate (APS) for molecular biology, 99% |
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65553 | Sisco Laboratories | 25 Gms | EUR 1.64 |
Description: Part A |
Glutathione Oxidized (GSSG) for molecular biology, 99.5% |
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22151 | Sisco Laboratories | 250 Mg | EUR 22.5 |
Description: Part B |
Cesium Chloride ultrapure for molecular biology, 99.9% |
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32355 | Sisco Laboratories | 25 Gms | EUR 18 |
Description: Part B |
Cesium Sulphate extrapure for molecular biology, 99.9% |
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57455 | Sisco Laboratories | 10 Gms | EUR 7.5 |
Description: Part B |
Guanidine Thiocyanate (GTC) for molecular biology, 99% |
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80272 | Sisco Laboratories | 100 Gms | EUR 5.47 |
Description: Part A |
Sucrose, GlenBiol™, suitable for molecular biology |
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GC3201 | Glentham Life Sciences | 1kg | EUR 41.25 |
Sucrose, GlenBiol™, suitable for molecular biology |
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GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
Formamide, GlenBiol™, suitable for molecular biology |
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GS9663 | Glentham Life Sciences | 100ml | EUR 46.83 |
Formamide, GlenBiol™, suitable for molecular biology |
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GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
Sodium Citrate Tribasic Dihydrate for molecular biology, 99% |
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67331 | Sisco Laboratories | 250 Gms | EUR 4.38 |
Description: Part A |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780 | Glentham Life Sciences | 2500ml | EUR 239.63 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
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GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
Diethyl Pyrocarbonate (DEPC) for molecular biology, 99% |
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46791 | Sisco Laboratories | 5 ml | EUR 9 |
Description: Part B |
Calcium Chloride Dihydrate for molecular biology, 99.5% |
|||
97080 | Sisco Laboratories | 100 Gms | EUR 2.12 |
Description: Part A |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659 | Glentham Life Sciences | 500ml | EUR 230.38 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
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GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
20X SSC Buffer pH 6.9-7.1 for molecular biology |
|||
12590 | Sisco Laboratories | 200 ml | EUR 2.48 |
Description: Part B |
Polyethylene Glycol 8000 (PEG 8000) for molecular biology |
|||
54866 | Sisco Laboratories | 500 Gms | EUR 11.97 |
Description: Part A |
Bromocresol Purple Sodium Salt for molecular biology |
|||
24836 | Sisco Laboratories | 5 Gms | EUR 2.05 |
Description: Part A |
Guanidine Hydrochloride (GHC) for molecular biology, 99.5% |
|||
45539 | Sisco Laboratories | 100 Gms | EUR 4.79 |
Description: Part A |
Phenol Tris Equilibrated for molecular biology w/ Stabilizer |
|||
12692 | Sisco Laboratories | 60 ml | EUR 10.13 |
Description: Part A |
Proteinase K ex. Tritirachium Album (Type A) for molecular biology, 30U/mg |
|||
49936 | Sisco Laboratories | 25 Mg | EUR 8.25 |
Description: Part B |
Magnesium Acetate Tetrahydrate for molecular biology, 99% |
|||
50488 | Sisco Laboratories | 100 Gms | EUR 2.74 |
Description: Part A |
Magnesium Chloride Hexahydrate for molecular biology, 99.5% |
|||
91417 | Sisco Laboratories | 250 Gms | EUR 4.45 |
Description: Part A |
20X Tris Acetate-SDS Buffer pH-8.3 for molecular biology |
|||
47804 | Sisco Laboratories | 500 ml | EUR 11.25 |
Description: Part B |
10X Tris Acetate-SDS Buffer pH-8.3 for molecular biology |
|||
98635 | Sisco Laboratories | 500 ml | EUR 6 |
Description: Part B |
Agarose Low EEO Superior Grade for molecular biology |
|||
23287 | Sisco Laboratories | 10 Gms | EUR 6 |
Description: Part B |
L-Lysine Monohydrate (base) for molecular biology, 99% |
|||
45976 | Sisco Laboratories | 25 Gms | EUR 37.63 |
Description: Part A |
Magnesium Sulphate Heptahydrate for molecular biology, 99.5% |
|||
74440 | Sisco Laboratories | 500 Gms | EUR 6.16 |
Description: Part A |
10X Tris-Glycine pH-8.3 Tank Buffer for molecular biology |
|||
87326 | Sisco Laboratories | 200 ml | EUR 6.3 |
Description: Part B |
Cesium Chloride extrapure AR for molecular biology, 99.9% |
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22966 | Sisco Laboratories | 25 Gms | EUR 14.63 |
Description: Part B |
N,N-Dimethylformamide (DMF) for molecular biology, 99.9% |
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24017 | Sisco Laboratories | 100 ml | EUR 3.08 |
Description: Part A |
EDTA Disodium Salt Dihydrate for molecular biology, 99.5% |
|||
43272 | Sisco Laboratories | 100 Gms | EUR 2.26 |
Description: Part A |
Saponin ex. Quillaja for molecular biology, 9% Sapogenin |
|||
80967 | Sisco Laboratories | 25 Gms | EUR 45 |
Description: Part B |
Chloroform : Isoamyl Alcohol (24:1) for molecular biology |
|||
85563 | Sisco Laboratories | 100 ml | EUR 8.48 |
Description: Part B |
1X Phosphate Buffered Saline (PBS) for molecular biology |
|||
95131 | Sisco Laboratories | 100 ml | EUR 3.98 |
Description: Part B |
Phenol Tris Equilibrated for molecular biology w/o Stabilizer |
|||
78348 | Sisco Laboratories | 100 ml | EUR 13.75 |
Description: Part A |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS3406 | Glentham Life Sciences | 2500ml | EUR 111.53 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
10X Phosphate Buffered Saline (PBS) for molecular biology |
|||
78529 | Sisco Laboratories | 100 ml | EUR 4.95 |
Description: Part B |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
|||
GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |