Molecular identification and performance evaluation of wild yeasts from different Ethiopian fermented products
Different types of yeasts and lactic acid bacteria dominate in spontaneously fermented products (food, beverages, and condiments) that are commonly consumed in Ethiopia. The aim of this study was to identify efficient fermentative yeasts from fermented foods, fermented beverages, honey and molasses using genotypic methods.
Out of the 70 samples tested, 180 distinct wild yeast isolates were recovered. A total of 23 isolates were selected for genomic analysis based on their basis of biomass yield, fermentation capacity, and leavening performance.
The nucleotide sequence analysis of the internal transcribed spacer ITS-5.8S rDNA region revealed that the indigenous yeast isolates had close relatedness to Saccharomyces cerevisiae, Candida humilis, Kazachstania bulderi, Pichia fermentans and Pichia kudriavzevii with greater than 97% nucleotide similarity.
The study shows a high diversity of indigenous wild yeasts in fermented products and that potent strains had higher biomass yield, good gas production and remarkable leavening capacity that indicates their inherent potential for use in the baking industry.
Keywords: Baker’s yeasts; Fermented products; ITS-5.8S rDNA; Spontaneous fermentation.
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Protein G Magnetic Beads |
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Anti-Flag magnetic beads |
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Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification. |
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Description: Anti-Flag magnetic beads is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody. It is recommended to use for co-immunoprecipitation and protein purification. |
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Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification. |
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Description: Anti-HA magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with high quality mouse IgG2b monoclonal antibody, used for co-immunoprecipitation and protein purification. |
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Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
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Description: Anti-Myc magnetic beads kit is based on hydroxyl magnetic beads covalently coupling with mouse IgG1 monoclonal antibody.it is recommended to use for co-immunoprecipitation and protein purification. |
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Protein A/G Magnetic Beads |
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Description: LV CryoOil 5 ml |
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Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
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Protein A/G Magnetic Beads for IP |
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Description: Protein A/G Magnetic Beads for immunoprecipitation (IP) are based on a biological nanosurface technology (S-TEC). Protein A/G coats the surface of super paramagnetic microspheres with high coating density up to 9.3 × 10^13 molecules/cm2. Compared to similar immune magnetic beads, our Protein A/G beads display much more antibody binding sites therefore using less magnetic beads per reaction and ultimately achiving greater cost-efficiency. Non-specific binding is reduced to an absolute low eliminating any detectable interference with your IP assays. The large, specific surface area of the beads greatly shortens the adsorption time and achieves complete antibody/antigen adsorption process in 10 minutes and complete total purification and precipitation in just 30 minutes. This product can be used on a wide variety of samples, such as in cell lysates, supernatants collected from cell secretion, serum, ascites, and other immune antigens. |
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Protein A Beads (1-2mg/ml) |
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Protein A Beads (1-2mg/ml) |
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100 BP DNA LADDER, 500UL PER KIT |
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Description: Bioscience Mol Bio; DNA Ladder |
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1 KB DNA LADDER, 500UL PER KIT |
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Description: Bioscience Mol Bio; DNA Ladder |
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Sodium hydroxide powder 5 M, 1000 ml |
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Amino Magnetic Particles |
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Description: Amino Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team. |
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Streptavidin Magnetic Particles |
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Streptavidin Magnetic Particles |
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Streptavidin Magnetic Particles |
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Biotin Magnetic Particles |
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Avidin Magnetic Particles |
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Avidin Magnetic Particles |
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ML 239 |
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| A8719-5 | ApexBio | 5 mg | EUR 166.8 |
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Description: ML239 is the best-in-class inhibitor of the breast cancer stem cells with an IC50= 1.16 µM. [1]ML239 was a selective inhibitor an IC50= 1.18 µM against HMLE_sh_Ecad. |
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ML-354 |
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| C3058-5 | ApexBio | 5 mg | EUR 174 |
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Description: IC50: 140 nMML-354 is an indole-based, proteinase-activated receptor (PAR) 4 antagonist with good potency and reasonable selectivity versus PAR1. PARs are a family of four G-protein-coupled receptors GPCRs (PAR1-PAR4) with several unique attributes, which are activated by serine proteases. |
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ML-356 |
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| C3354-5 | ApexBio | 5 mg | EUR 474 |
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Description: ML-356 is an inhibitor of the thioesterase domain of fatty acid synthase with IC50 value of 334 nM.Fatty acid synthase (FAS) is a multi-enzyme protein that catalyzes fatty acid synthesis, fatty acids are related to energy production and storage, cellular structure ,hormones biosynthesis |
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ML-264 |
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| C3474-5 | ApexBio | 5 mg | EUR 195.6 |
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Description: ML264, a potent and selective inhibitor of kruppel-like factor 5 (KLF5), inhibits the growth of colorectal cancer. |
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Molecular Targets of Natural Products for Chondroprotection in Destructive Joint Diseases
Osteoarthritis (OA) is the most common type of arthritis that occurs in an aged population. It affects any joints in the body and degenerates the articular cartilage and the subchondral bone. Despite the pathophysiology of OA being different, cartilage resorption is still a symbol of osteoarthritis. Matrix metalloproteinases (MMPs) are important proteolytic enzymes that degrade extra-cellular matrix proteins (ECM) in the body.
MMPs contribute to the turnover of cartilage and its break down; their levels have increased in the joint tissues of OA patients. Application of chondroprotective drugs neutralize the activities of MMPs. Natural products derived from herbs and plants developed as traditional medicine have been paid attention to, due to their potential biological effects.
The therapeutic value of natural products in OA has increased in reputation due to their clinical impact and insignificant side effects. Several MMPs inhibitor have been used as therapeutic drugs, for a long time.
Recently, different types of compounds were reviewed for their biological activities. In this review, we summarize numerous natural products for the development of MMPs inhibitors in arthritic diseases and describe the major signaling targets that were involved for the treatments of these destructive joint diseases.
Keywords: MMPs; arthritis; chondroprotection; natural products; signaling pathways
Catalytic Mechanism and Product Specificity of Protein Arginine Methyltransferase PRMT7: A Study from QM/MM Molecular Dynamics and Free Energy Simulations
QM/MM molecular dynamics and potential of mean force (PMF) free-energy simulations are performed for wild-type PRMT7 and E172Q, E181Q, and Q329A mutants in this work, and the catalytic mechanism, product specificity, and the role of key residues for the PRMT7 activity are investigated. The main strategies of PRMT7 in reducing the activation barrier for methyl transfer that are found in this study include (1) formation of reactive (near attack) conformations for the substrate Arg, (2) strengthening the active-site interactions at the transition state, and (3) generation of more effective nucleophiles by changing charge distributions on the target Arg through active-site interactions.
- More importantly, it is shown that it is a combination of these different factors that determines the PRMT7 methylation activity and substrate/product specificity.
- By taking these factors into consideration, it is possible to provide explanations for the observed effects of some mutations. For E172Q, E181Q, and Q329A, the simulation results suggest that E172Q has the least activity among the three mutants.
- The free energy barrier increases by 7 and 3 kcal/mol, respectively, as a result of the E181 → Q and Q329 → A mutations. The results showed that PRMT7 has a preference of adding a methyl group to the ω-guanidino nitrogen Nη2 atom of the substrate Arg and that the second methylation reactions cannot occur, which are consistent with previous investigations.
5-min. Genomic DNA Prep for PCR |
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| P930-500 | 101Bio | - | Ask for price |
Genomic DNA - Human Male |
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Genomic DNA - Rabbit Male |
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Genomic DNA - Porcine Female |
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| D1934999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Bovine Male |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Bovine Female |
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| D1B34999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Chicken Male |
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| D1C34999-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Chicken Female |
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| D1C34999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Hamster Male |
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| D1H34999-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Hamster Female |
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| D1H34999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Horse Male |
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| D1O34999-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Horse Female |
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| D1O34999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA Isolation Kit |
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| 55R-1362 | Fitzgerald | 50 assays | EUR 462 |
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Description: Genomic DNA Isolation Kit for use in the research laboratory |
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Blood Genomic DNA Kit |
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| abx098868-50rxns | Abbexa | 50 rxns | EUR 326.4 |
Plant Genomic DNA Kit |
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| 20-abx098077 | Abbexa |
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Bacteria Genomic DNA Kit |
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| 20-abx098080 | Abbexa |
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Micro Genomic DNA Kit |
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| 20-abx098242 | Abbexa |
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Plant Genomic DNA Kit |
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| abx294004-50preps | Abbexa | 50 preps | EUR 477.6 |
Animal Genomic DNA Kit |
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| abx294005-100preps | Abbexa | 100 preps | EUR 627.6 |
Animal Genomic DNA Kit |
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| abx294005-50preps | Abbexa | 50 preps | EUR 477.6 |
Genomic DNA Isolation Kit |
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| K281-50 | Biovision | EUR 326.4 | |
Soil Genomic DNA Kit |
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| K1411-250 | Biovision | EUR 673.2 | |
Soil Genomic DNA Kit |
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| K1411-50 | Biovision | EUR 379.2 | |
Insect Genomic DNA Kit |
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| K1412-250 | Biovision | EUR 673.2 | |
Insect Genomic DNA Kit |
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| K1412-50 | Biovision | EUR 379.2 | |
Mollusc Genomic DNA Kit |
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| K1413-250 | Biovision | EUR 673.2 | |
Mollusc Genomic DNA Kit |
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| K1413-50 | Biovision | EUR 379.2 | |
Yeast Genomic DNA Kit |
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| K1414-250 | Biovision | EUR 673.2 | |
Yeast Genomic DNA Kit |
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| K1414-50 | Biovision | EUR 379.2 | |
Fungal Genomic DNA Kit |
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Fungal Genomic DNA Kit |
|||
| K1415-50 | Biovision | EUR 379.2 | |
Genomic DNA Isolation Kit |
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| K2118-50 | ApexBio | 50 assays | EUR 385.2 |
Human Brain Genomic DNA |
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| X11001-1 | EpiGentek | 10 ul | EUR 77 |
Human Brain Genomic DNA |
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| X11001 | EpiGentek |
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Limit of Stray Light Std (340nm) Sodium Nitrite |
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| RSPEC-CP0361 | Scientific Laboratory Supplies | 100ML | EUR 371.64 |
Limit of Stray Light Std 220nm Na Iodide |
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| RSPEC-CP0371 | Scientific Laboratory Supplies | 100ML | EUR 201.78 |
Genomic DNA - Cynomolgus Monkey Male |
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| D1534999-Cy-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Cynomolgus Monkey Female |
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| D1534999-Cy-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Rhesus Monkey Male |
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| D1534999-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Rhesus Monkey Female |
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| D1534999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Guinea Pig Male |
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| D1G34999-G01 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA - Guinea Pig Female |
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| D1G34999-G02 | Biochain | 100 ug | EUR 225.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Genomic DNA Extraction and Purification |
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| EP10012 | Neuromics | 100 Tests | EUR 606 |
FFPE Tissue Genomic DNA Kit |
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| abx098866-50rxns | Abbexa | 50 rxns | EUR 326.4 |
Plant Genomic DNA extraction buffer |
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| 20-abx098075 | Abbexa |
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Marine Animal Genomic DNA Kit |
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| 20-abx098079 | Abbexa |
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ISOLATE II Genomic DNA Kit |
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| BIO-52065 | Bioline | 10 preps | Ask for price |
ISOLATE II Genomic DNA Kit |
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| BIO-52066 | Bioline | 50 preps | Ask for price |
ISOLATE II Genomic DNA Kit |
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| BIO-52067 | Bioline | 250 preps | Ask for price |
Genomic DNA Extraction KitAnimal Tissue |
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| FYG102-100P | Yeastern Biotech | 100 Preps | Ask for price |
Genomic DNA Extraction KitAnimal Tissue |
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| FYG102-300P | Yeastern Biotech | 300 Preps | Ask for price |
Genomic DNA Extraction KitAnimal Tissue |
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| FYG102-50P | Yeastern Biotech | 50 Preps | Ask for price |
Genomic DNA Extraction Maxi KitPlant |
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| FYG104-10P | Yeastern Biotech | 10 Preps | Ask for price |
Genomic DNA Extraction Maxi KitPlant |
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| FYG104-25P | Yeastern Biotech | 25 Preps | Ask for price |
AccuRT Genomic DNA Removal Kit |
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| G488 | ABM | 200 Reactions | EUR 98.4 |
Bacterial Genomic DNA Isolation Kit |
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| K309-100 | Biovision | EUR 451.2 | |
Zenoquick Genomic DNA Purification Kit |
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| Z2003-200 | GenDepot | 200 preps | EUR 757.2 |
Zenoprep Genomic DNA Purification Kit |
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| Z2004-400 | GenDepot | 400 preps | EUR 660 |
Sera-Xtracta Genomic DNA Kit |
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| 29429140 | Scientific Laboratory Supplies | EACH | EUR 380.76 |
Limit of Stray Light Blank 340nm for use w/ Na Nitrite Std |
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| RSPEC-CP0362 | Scientific Laboratory Supplies | 100ML | EUR 201.78 |
Limit of Stray Light Blank 220nm for use w/ Na Iodide Std |
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| RSPEC-CP0372 | Scientific Laboratory Supplies | 100ML | EUR 167.58 |
DNA Methylation Detection Kit |
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| K5082100 | Biochain | 1 kit | EUR 412.8 |
100 BP DNA LADDER, 500UL PER KIT |
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| M-DNA-100BP | CORNING | 1/pk | EUR 87.6 |
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Description: Bioscience Mol Bio; DNA Ladder |
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1 KB DNA LADDER, 500UL PER KIT |
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| M-DNA-1KB | CORNING | 1/pk | EUR 84 |
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Description: Bioscience Mol Bio; DNA Ladder |
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Rapid Bacteria Genomic DNA Isolation Kit |
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| BS8225 | Bio Basic | 50Preps | EUR 91.97 |
Rapid Yeast Genomic DNA Extraction Kit |
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| BS8227 | Bio Basic | 50Preps | EUR 91.97 |
Rapid Blood Genomic DNA Extraction Kit |
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| BT4782 | Bio Basic | 50Preps | EUR 91.97 |
Genomic DNA Kit (No RNase A) |
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| 20-abx098862 | Abbexa |
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Rapid Animal Genomic DNA Extraction Kit |
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| AT4780 | Bio Basic | 10Preps | EUR 82.84 |
Rapid Animal Genomic DNA Extraction Kit |
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| AT4781 | Bio Basic | 50Preps | EUR 151.36 |
Rapid Animal Genomic DNA Extraction Kit |
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| AT4782 | Bio Basic | 250preps | EUR 425.4 |
Mammalian Cell Genomic DNA Isolation Kit |
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| K967-100 | Biovision | EUR 457.2 | |
Rapid Fungal Genomic DNA Isolation Kit |
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| FT71415 | Bio Basic | 50Preps | EUR 91.97 |
Genomic DNA Extraction Mini KitPlant Tissue |
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| FYG103-100P | Yeastern Biotech | 100 Preps | Ask for price |