Molecular identification and performance evaluation of wild yeasts from different Ethiopian

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Molecular identification and performance evaluation of wild yeasts from different Ethiopian fermented products

Different types of yeasts and lactic acid bacteria dominate in spontaneously fermented products (food, beverages, and condiments) that are commonly consumed in Ethiopia. The aim of this study was to identify efficient fermentative yeasts from fermented foods, fermented beverages, honey and molasses using genotypic methods.
Out of the 70 samples tested, 180 distinct wild yeast isolates were recovered. A total of 23 isolates were selected for genomic analysis based on their basis of biomass yield, fermentation capacity, and leavening performance.
The nucleotide sequence analysis of the internal transcribed spacer ITS-5.8S rDNA region revealed that the indigenous yeast isolates had close relatedness to Saccharomyces cerevisiaeCandida humilisKazachstania bulderiPichia fermentans and Pichia kudriavzevii with greater than 97% nucleotide similarity.
The study shows a high diversity of indigenous wild yeasts in fermented products and that potent strains had higher biomass yield, good gas production and remarkable leavening capacity that indicates their inherent potential for use in the baking industry.
Keywords: Baker’s yeasts; Fermented products; ITS-5.8S rDNA; Spontaneous fermentation.
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Amylose BlackPearl® (Magnetic Beads)

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Human ANGPT2-coupled Magnetic Beads

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Molecular Targets of Natural Products for Chondroprotection in Destructive Joint Diseases

Osteoarthritis (OA) is the most common type of arthritis that occurs in an aged population. It affects any joints in the body and degenerates the articular cartilage and the subchondral bone. Despite the pathophysiology of OA being different, cartilage resorption is still a symbol of osteoarthritis. Matrix metalloproteinases (MMPs) are important proteolytic enzymes that degrade extra-cellular matrix proteins (ECM) in the body.

MMPs contribute to the turnover of cartilage and its break down; their levels have increased in the joint tissues of OA patients. Application of chondroprotective drugs neutralize the activities of MMPs. Natural products derived from herbs and plants developed as traditional medicine have been paid attention to, due to their potential biological effects.

The therapeutic value of natural products in OA has increased in reputation due to their clinical impact and insignificant side effects. Several MMPs inhibitor have been used as therapeutic drugs, for a long time.

Recently, different types of compounds were reviewed for their biological activities. In this review, we summarize numerous natural products for the development of MMPs inhibitors in arthritic diseases and describe the major signaling targets that were involved for the treatments of these destructive joint diseases.

Keywords: MMPs; arthritis; chondroprotection; natural products; signaling pathways

Catalytic Mechanism and Product Specificity of Protein Arginine Methyltransferase PRMT7: A Study from QM/MM Molecular Dynamics and Free Energy Simulations

QM/MM molecular dynamics and potential of mean force (PMF) free-energy simulations are performed for wild-type PRMT7 and E172Q, E181Q, and Q329A mutants in this work, and the catalytic mechanism, product specificity, and the role of key residues for the PRMT7 activity are investigated. The main strategies of PRMT7 in reducing the activation barrier for methyl transfer that are found in this study include (1) formation of reactive (near attack) conformations for the substrate Arg, (2) strengthening the active-site interactions at the transition state, and (3) generation of more effective nucleophiles by changing charge distributions on the target Arg through active-site interactions.
  1. More importantly, it is shown that it is a combination of these different factors that determines the PRMT7 methylation activity and substrate/product specificity.
  2. By taking these factors into consideration, it is possible to provide explanations for the observed effects of some mutations. For E172Q, E181Q, and Q329A, the simulation results suggest that E172Q has the least activity among the three mutants.
  3. The free energy barrier increases by 7 and 3 kcal/mol, respectively, as a result of the E181 → Q and Q329 → A mutations. The results showed that PRMT7 has a preference of adding a methyl group to the ω-guanidino nitrogen Nη2 atom of the substrate Arg and that the second methylation reactions cannot occur, which are consistent with previous investigations.

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Propidium Iodide for Apoptosis Detection

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Soil Genomic DNA Kit

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Soil Genomic DNA Kit

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Blood Genomic DNA Kit

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Yeast Genomic DNA Kit

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DNase Detection Kit

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DNase Detection Kit

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Animal Genomic DNA Kit

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Human CD9 Capture Beads for Flow Detection

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Human CD81 Capture Beads for Flow Detection

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Mouse CD63 Capture Beads for Flow Detection

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Description: CD63 (Clone NVG-2) Capture Beads

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Multi-Species Creatinine Detection Kit for Urine

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Marine Animal Genomic DNA Kit

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MINI GENOMIC DNA KIT-TISSUE

IB47221 50 PREP KIT
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IB47222 300 PREP KIT
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Human CD326 (EpCAM) Capture Beads for Flow Detection

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EUR 387.6

Sera-Xtracta Genomic DNA Kit

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Control Genomic DNA - Mouse Male

D1334999-G01 100 ug
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