Engineering Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine to pyridoxine.

      No Comments on Engineering Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine to pyridoxine.
allelebio

Until now, pyridoxine (PN), the most common vitamer B6 comes to animals and humans, are chemically synthesized for commercial purposes. Thus, the development of microbial fermentation process is very attractive to the biotechnology industry.

More recently, we construct strain Bacillus subtilis were formed in a significant number of PN by non-native deoxyxylulose 5′-phosphate- (DXP) -dependent pathway of vitamin B6. Here we report on the optimization of the condensation reaction of this pathway consisting of 4-hydroxy-L-threonine-phosphate dehydrogenase pDXA, which pyridoxine 5′-phosphate synthase original PdxJ and DXP synthase, DXS. To enable eat high amounts of 4-hydroxy-threonine (4-HO-Thr) which can be converted to a PN by B. subtilis overexpressing pDXA and PdxJ, we first adjusted the bacteria to tolerate antimetabolite 4-HO-Thr. Adapted bacteria produced 28-34mg / l PN of 4-HO-Thr whereas the wild-type parent only produced 12 mg / l PN.

Moreover, by expressing alleles differ in their pdxJ pDXA and adapted strains we identified a better combination of enzymes pDXA and PdxJ than previously reported, and the resulting strain produced 65 mg / l PN. To further improve productivity mutants were isolated that efficiently retrieve and convert deoxyxylulose (DX) to DXP, which is inserted into the PN. Although the mutant highly efficient for converting low amounts of exogenous DX, at a higher level of DX they performed only slightly better. This study found some enzymes with promiscuous activities and revealed that the host’s metabolic pathways compete with heterologous pathway for 4-HO-Thr. In addition, the study showed that B. subtilis genome is quite flexible with respect to adaptive mutation, the property, which is very important for strain engineering.


Qualitative tests compared with the gold standard quantitative test. G6PDd higher prevalence of RDT (8.6%) compared with the quantitative assay (6.8%), using <60% threshold of activity. RDT performed optimally at <threshold of 60% and showed high sensitivity (≥ 90%) and negative predictive values ​​(100%) in the three threshold of activity (under 60%, 30% and 40%).

G202A allele frequency was 6.4%, 7.9%, and 6.8% among women, men, and overall, respectively. Notably, more than half of G202A homo / child hemizygous states ≥ 60% activity of the enzyme. Overall, CareStart ™ G6PD RDT seems to be a feasible screening test to accurately identify individuals with enzyme activity below 60%. G6PDd low prevalence in all three diagnostic modalities and the absence of severe deficiency in our study suggests that there is little resistance to the use of a single dose of primaquine in this region.

Engineering Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine to pyridoxine.
Engineering Bacillus subtilis for the conversion of the antimetabolite 4-hydroxy-l-threonine to pyridoxine.

Optimization of DNA amplification hexaplex of short tandem repeat loci amelogenin.

An automated system of DNA profiling based multiplex amplification of highly polymorphic short tandem Repeat (STR) loci amelogenin markers and developed. Five STR loci with overlapping allele size range has been used in the multiplex amplification, including HUMD1S103, HUMTH01, HUMD21S11, HUMD18S51, and HUMFIBRA.

p38
MBS8533658-01mLAF405S 0.1mL(AF405S)
EUR 565

p38
MBS8533658-01mLAF610 0.1mL(AF610)
EUR 565

p38
MBS8533658-01mLAF635 0.1mL(AF635)
EUR 565

P38 mAb
MBS435304-005mL 0.05mL
EUR 415

P38 mAb
MBS435304-01mL 0.1mL
EUR 660

P38 mAb
MBS435304-5x01mL 5x0.1mL
EUR 2655

p38 MAPK
E8ET1702-65 100ul
EUR 275
Description: Available in various conjugation types.

Mxi2, p38
MBS603655-01mg 0.1mg
EUR 820

Mxi2, p38
MBS603655-5x01mg 5x0.1mg
EUR 3530

p38 MAPK
MBS8534372-01mL 0.1mL
EUR 345

p38 MAPK
MBS8534372-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 MAPK
MBS8534372-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 MAPK
MBS8534372-01mLAF610 0.1mL(AF610)
EUR 565

p38 MAPK
MBS8534372-01mLAF635 0.1mL(AF635)
EUR 565

p38 protein
E20-80078 20ug
EUR 275
Description: N/A

p38 protein
E20-80079 20ug
EUR 275
Description: N/A

p38 protein
MBS8526800-002mg 0.02mg
EUR 345

p38 protein
MBS8526800-5x002mg 5x0.02mg
EUR 1410

p38 protein
MBS8526801-002mg 0.02mg
EUR 345

p38 protein
MBS8526801-5x002mg 5x0.02mg
EUR 1410

p38 Antibody
20-abx007541
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

p38 antibody
70R-51505 100 ul
EUR 242
Description: Purified Polyclonal p38 antibody

p38 antibody
70R-51506 100 ul
EUR 286
Description: Purified Polyclonal p38 antibody

p38 Antibody
35478 100ul
EUR 479

p38 Antibody
35478-100ul 100ul
EUR 468

p38 Antibody
20-abx133223
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody
48644 100ul
EUR 499

p38 Antibody
48644-100ul 100ul
EUR 399.6

p38 Antibody
48644-50ul 50ul
EUR 286.8

p38 Antibody
20-abx141038
  • Ask for price
  • Ask for price
  • Ask for price
  • 30 ul
  • 100 ul
  • 200 ul

p38 Antibody
B7178-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B7178-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0798-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-100ul 100μl
EUR 217
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
B0799-50ul 50μl
EUR 143.5
Description: p38 Rabbit Polyclonal Antibody

p38 Antibody
abx236076-100g 100 µg
EUR 350

p38 Antibody
abx236076-100ug 100 ug
EUR 577.2

p38 Antibody
E38PA4721 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5060 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA5094 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA7126 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
E38PA3186 100ul
EUR 225
Description: Available in various conjugation types.

p38 Antibody
MBS5316121-01mL 0.1mL
EUR 460

p38 Antibody
MBS5316121-5x01mL 5x0.1mL
EUR 1920

p38 Antibody
MBS5316122-01mL 0.1mL
EUR 535

p38 Antibody
MBS5316122-5x01mL 5x0.1mL
EUR 2270

p38 Antibody
MBS8002013-0025mL 0.025mL
EUR 315

p38 Antibody
MBS8002013-5x0025mL 5x0.025mL
EUR 890

p38 Antibody
MBS805089-01mL 0.1mL
EUR 425

p38 Antibody
MBS805089-5x01mL 5x0.1mL
EUR 1350

p38 Antibody
MBS806203-0025mL 0.025mL
EUR 320

p38 Antibody
MBS8581787-01mL 0.1mL
EUR 305

p38 Antibody
MBS8581787-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8581787-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8581787-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8581787-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8584173-01mL 0.1mL
EUR 305

p38 Antibody
MBS8584173-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8584173-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8584173-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8584173-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582665-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582665-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582665-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582665-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582665-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582905-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582905-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582905-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582905-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582905-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS8582923-01mL 0.1mL
EUR 305

p38 Antibody
MBS8582923-01mLAF405L 0.1mL(AF405L)
EUR 465

p38 Antibody
MBS8582923-01mLAF405S 0.1mL(AF405S)
EUR 465

p38 Antibody
MBS8582923-01mLAF610 0.1mL(AF610)
EUR 465

p38 Antibody
MBS8582923-01mLAF635 0.1mL(AF635)
EUR 465

p38 Antibody
MBS9508977-005mL 0.05mL
EUR 285

p38 Antibody
MBS9508977-01mL 0.1mL
EUR 385

p38 Antibody
MBS9508977-5x01mL 5x0.1mL
EUR 1590

p38 Antibody
MBS9409774-01mL 0.1mL
EUR 420

p38 Antibody
MBS9409774-5x01mL 5x0.1mL
EUR 1740

p38 Antibody
MBS9435492-005mL 0.05mL
EUR 325

p38 Antibody
MBS9435492-01mL 0.1mL
EUR 435

p38 Antibody
MBS9435492-5x01mL 5x0.1mL
EUR 1810

p38 Antibody
SPC-172D 0.1ml
EUR 161
Description: Rabbit Anti-Human p38 Polyclonal

p38 Antibody
SPC-172S 0.012ml Ask for price
Description: Rabbit Anti-Human p38 Polyclonal

p38 Substrate
MBS517838-1mg 1mg
EUR 205

p38 Substrate
MBS517838-5x1mg 5x1mg
EUR 675

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-10Blots 10Blots
EUR 430

MAP Kinase p38, Control Cell Extracts (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS657552-5x10Blots 5x10Blots
EUR 1785

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-01mL 0.1mL
EUR 695

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS633592-5x01mL 5x0.1mL
EUR 2985

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-02mL 0.2mL
EUR 660

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS632290-5x02mL 5x0.2mL
EUR 2810

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-02mL 0.2mL
EUR 650

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-06mL 0.6mL
EUR 1175

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS630803-5x06mL 5x0.6mL
EUR 5140

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-10Blots 10Blots
EUR 875

MAP Kinase p38, phosphorylated (Thr180, Tyr182) (Mitogen Activated Protein Kinase p38, MAPK p38)
MBS627818-5x10Blots 5x10Blots
EUR 3785

SB-203580 p38 MAPK(p38 MAP kinase) Inhibitor
E1B1863 1mg
EUR 85

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-1mg 1mg
EUR 180

SB-203580 p38 MAPK (p38 MAP kinase) Inhibitor
MBS8579825-5x1mg 5x1mg
EUR 660

p38 (411-425) M.leprae
MBS8246858-10mg 10mg
EUR 675

p38 (411-425) M.leprae
MBS8246858-1mg 1mg
EUR 255

p38 (411-425) M.leprae
MBS8246858-5mg 5mg
EUR 480

p38 (411-425) M.leprae
MBS8246858-5x10mg 5x10mg
EUR 2955

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-04mL 0.4mL
EUR 670

MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38) (Agarose)
MBS631660-5x04mL 5x0.4mL
EUR 2870

Anti - p38-a
MBS684252-002mL 0.02mL
EUR 270

Anti - p38-a
MBS684252-005mL 0.05mL
EUR 485

Anti - p38-a
MBS684252-01mL 0.1mL
EUR 720

Anti - p38-a
MBS684252-5x01mL 5x0.1mL
EUR 2925

Anti - p38-b
MBS684253-002mL 0.02mL
EUR 270

Anti - p38-b
MBS684253-005mL 0.05mL
EUR 485

Anti - p38-b
MBS684253-01mL 0.1mL
EUR 720

Anti - p38-b
MBS684253-5x01mL 5x0.1mL
EUR 2925

Anti - p38-g
MBS684254-002mL 0.02mL
EUR 270

Anti - p38-g
MBS684254-005mL 0.05mL
EUR 485

Anti - p38-g
MBS684254-01mL 0.1mL
EUR 720

Anti - p38-g
MBS684254-5x01mL 5x0.1mL
EUR 2925

Anti - p38-d
MBS684255-002mL 0.02mL
EUR 270

Anti - p38-d
MBS684255-005mL 0.05mL
EUR 485

Anti - p38-d
MBS684255-01mL 0.1mL
EUR 720

Anti - p38-d
MBS684255-5x01mL 5x0.1mL
EUR 2925

p38 Mouse mAb
E2220879 100ul
EUR 225
Description: Available in various conjugation types.

p38 Mouse mAb
E2222580 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

p38 Mouse mAb
MBS8539086-01mL 0.1mL
EUR 305

p38 Mouse mAb
MBS8539086-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF610 0.1mL(AF610)
EUR 565

p38 Mouse mAb
MBS8539086-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
E2340697 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2380881 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2381051 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
E2R25239 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
E2R27123 100μl
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
E38A4061 100ug/100ul
EUR 255

p38 Rabbit pAb
E2251338 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit pAb
E2251719 100ul
EUR 225
Description: Available in various conjugation types.

p38 Rabbit mAb
MBS4754198-01mL 0.1mL
EUR 450

p38 Rabbit mAb
MBS4754198-5x01mL 5x0.1mL
EUR 1540

p38 Rabbit mAb
MBS4752931-01mL 0.1mL
EUR 450

p38 Rabbit mAb
MBS4752931-5x01mL 5x0.1mL
EUR 1540

p38 HOG Kinase
MBS395428-025mg 0.25mg
EUR 425

p38 HOG Kinase
MBS395428-5x025mg 5x0.25mg
EUR 1760

p38 Rabbit pAb
MBS8542346-01mL 0.1mL
EUR 305

p38 Rabbit pAb
MBS8542346-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb
MBS8542346-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb
MBS8542346-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb
MBS8542346-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
MBS8542516-01mL 0.1mL
EUR 305

p38 Rabbit pAb
MBS8542516-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb
MBS8542516-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb
MBS8542516-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb
MBS8542516-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
MBS8539985-01mL 0.1mL
EUR 305

p38 Rabbit pAb
MBS8539985-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb
MBS8539985-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb
MBS8539985-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb
MBS8539985-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
MBS8541727-01mL 0.1mL
EUR 305

p38 Rabbit pAb
MBS8541727-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb
MBS8541727-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb
MBS8541727-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb
MBS8541727-01mLAF635 0.1mL(AF635)
EUR 565

p38 Rabbit pAb
MBS8540327-01mL 0.1mL
EUR 305

p38 Rabbit pAb
MBS8540327-01mLAF405L 0.1mL(AF405L)
EUR 565

p38 Rabbit pAb
MBS8540327-01mLAF405S 0.1mL(AF405S)
EUR 565

p38 Rabbit pAb
MBS8540327-01mLAF610 0.1mL(AF610)
EUR 565

p38 Rabbit pAb
MBS8540327-01mLAF635 0.1mL(AF635)
EUR 565

anti-Crk p38
YF-PA23509 50 ul
EUR 400.8
Description: Mouse polyclonal to Crk p38

anti-Crk p38
YF-PA11088 50 ul
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38
YF-PA11089 50 ug
EUR 435.6
Description: Mouse polyclonal to Crk p38

anti-Crk p38
YF-PA11090 100 ug
EUR 483.6
Description: Rabbit polyclonal to Crk p38

p38 beta, Active
7763-100 each
EUR 5214

p38 beta, Active
7763-5 each
EUR 444

p38 beta protein
30R-2875 5 ug
EUR 425
Description: Purified recombinant Human p38 beta protein

Crk p38 Antibody
AF6323 200ul
EUR 420

Crk p38 Antibody
AF6323-100ul 100ul
EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Crk p38 Antibody
AF6323-200ul 200ul
EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

p38 (C20) Antibody
MBS440021-02mg 0.2mg
EUR 285

p38 (C20) Antibody
MBS440021-5x02mg 5x0.2mg
EUR 1185

p38 Antibody: HRP
MBS805179-01mL 0.1mL
EUR 455

p38 Antibody: HRP
MBS805179-5x01mL 5x0.1mL
EUR 1505

p38 Antibody: RPE
MBS805299-01mL 0.1mL
EUR 465

p38 Antibody: RPE
MBS805299-5x01mL 5x0.1mL
EUR 1535

p38 Hog Antibody
P9809 100ug
EUR 259

p38 Antibody: APC
SPC-172D-APC 0.1ml
EUR 197.4
Description: Rabbit Anti-Human p38 Polyclonal

p38 Antibody: HRP
SPC-172D-HRP 0.1ml
EUR 189
Description: Rabbit Anti-Human p38 Polyclonal

One primer for each locus labeled with a fluorescent dye (fluorescein) that allows detection at a single wavelength ALF DNA Sequencer (Pharmacia Biotech). As part of a detailed evaluation of the suitability of the system for use forensic hexaplex routine, the effect of variations in amplification parameters on the efficiency of the system checked. polymerase chain reaction amplification conditions are optimized to provide specific, strong amplification of forensic samples.

Leave a Reply

Your email address will not be published. Required fields are marked *